IN VITRO STUDY OF DIFFERENT SAMPLES OF 316 STAINLESS STEEL AS ORTHOPAEDIC BIOCOMPATIBLE MATERIALS
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Olayide R. Adetunji
Federal University of Agriculture, Abeokuta
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- Abstract
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This study examines the in vitro behaviour of different prepared samples of 316 stainless steel materials as orthopaedic biocompatible materials. In this research, three samples of 316 stainless steels (SS) were prepared for in vitro assessments. Sample 1 was 316SS alloy, sample 2 heat treated 316SS and sample 3 heat treated 316SS coated with graphene. Ringer solution was prepared as the simulated body fluid (SBF) using standard reagents and the samples were preserved in the SBF for ten (10) weeks in a water bath at 370C. Electrochemical test was carried out using potentiodynamic polarization method (PDP). Biological assays were carried out to determine turbidity and colony forming units (CFUs) after 60 days of culturing using McFarland standard test. The PDP showed that sample 3 had the better corrosion resistance with the highest negative corrosion potential (Ecorr) of -0.3940 (V). It also produced the most turbidity among the three samples which were 5.7, 3.6 and 2.2 and CFUs of 2.8 x 108, 3.3 x 108 and 4.4 x 108 cells/mL, respectively at the fortieth, fiftieth and sixtieth days. This indicated a lower corrosion rate and also the ability to allow organisms proliferation compared to the other prepared samples.
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